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学者姓名：高景峰
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摘要 ：
Surfactants could influence sludge morphology and disinfectants were linked to antibiotic resistance genes (ARGs). Thus, the response of activated sludge and ARGs to long-term quaternary ammonium compounds (QACs) exposure required further investigation, which is a popular surfactant and disinfectant. Here, three sequencing batch reactors were fed with 5 mg/L most frequently detected QACs (dodecyl trimethyl ammonium chloride (ATMAC C12), dodecyl benzyl dimethyl ammonium chloride (BAC C12) and didodecyl dimethyl ammonium chloride (DADMAC C12)) for 180 d. The long-term inhibitory effect on denitrification ranked: DADMAC C12 > BAC C12 > ATMAC C12. Besides, obvious granular sludge promoted by the increase of alpha-Helix/(8-Sheet + Random coil) appeared in DADMAC C12 system. Moreover, intracellular ARGs increased when denitrification systems encountered QACs acutely but decreased in systems chronically exposed to QACs. Although replication and repair metabolism in ATMAC C12 system was higher, ATMAC C12 significantly promoted proliferation of extracellular ARGs. It was noteworthy that the propagation risk of extracellular ARGs in sludge increased significantly during sludge granulation process, and intracellular sul2 genes in sludge and water both increased with the granular diameter in DADMAC C12 system. The universal utilization of QACs may enhance antibiotic resistance of bacteria in wastewater treatment plants, deserving more attention.
关键词 ：
Intracellular and extracellular antibiotic  Intracellular and extracellular antibiotic  Denitrification system Denitrification system Bacterial community structure Bacterial community structure resistance genes resistance genes Sludge granulation Sludge granulation Quaternary ammonium compounds Quaternary ammonium compounds
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| GB/T 7714 | Zhao, Mingyan , Gao, Jingfeng , Zhang, Haoran et al. Quaternary ammonium compounds promoted anoxic sludge granulation and altered propagation risk of intracellular and extracellular antibiotic resistance genes [J]. | JOURNAL OF HAZARDOUS MATERIALS , 2023 , 445 . |
| MLA | Zhao, Mingyan et al. "Quaternary ammonium compounds promoted anoxic sludge granulation and altered propagation risk of intracellular and extracellular antibiotic resistance genes" . | JOURNAL OF HAZARDOUS MATERIALS 445 (2023) . |
| APA | Zhao, Mingyan , Gao, Jingfeng , Zhang, Haoran , Cui, Yingchao , Wang, Zhiqi , Zhao, Yifan et al. Quaternary ammonium compounds promoted anoxic sludge granulation and altered propagation risk of intracellular and extracellular antibiotic resistance genes . | JOURNAL OF HAZARDOUS MATERIALS , 2023 , 445 . |
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摘要 ：
Achieving stable partial nitrification (PN) are the basic guarantee of anaerobic ammonia oxidation (anammox) process. However, there is no recognized feasible method for rapidly achieving stable PN in municipal wastewater, and further research is necessary. In this study, paracetamol was proved as an effective nitrite oxidation inhibitor through long-term experiment lasting 392 days in three systems (floc sludge, granular sludge, biofilm). PN of low-strength ammonium wastewater was rapidly achieved within 28 days and maintained for about 80 days after paracetamol dose, whereas PN recovered again within 10 days and sustained over 150 days under paracetamol dose combined with low dissolved oxygen strategy. The inhibition effect of paracetamol on NOB was almost not affected by biomass growth modes. QPCR and high-throughput sequencing showed that Nitrospira (Oligotype 1) was inhibited by paracetamol, and PICURSt2 analysis indicated its key enzyme NXR was significantly downregulated after paracetamol treatment, which were the key reasons of PN start-up in three systems. The emergence and adaptation of Nitrotoga was the main reason for the instability of PN. This study proved that paracetamol could be used as a novel nitrite oxidation inhibitor, and provided a possibility to achieve the mainstream PN and anammox for treating wastewater with low-strength ammonium.
关键词 ：
Low-strength ammonium wastewater Low-strength ammonium wastewater Bacterial community structure Bacterial community structure Biomass growth mode Biomass growth mode Paracetamol Paracetamol Partial nitrification Partial nitrification
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| GB/T 7714 | Cui, Yingchao , Gao, Jingfeng , Wu, Zejie et al. Inhibition of Nitrospira and Nitrotoga by paracetamol achieved the rapid start-up and long-term stable operation of partial nitrification for low-strength ammonium wastewater [J]. | CHEMICAL ENGINEERING JOURNAL , 2023 , 454 . |
| MLA | Cui, Yingchao et al. "Inhibition of Nitrospira and Nitrotoga by paracetamol achieved the rapid start-up and long-term stable operation of partial nitrification for low-strength ammonium wastewater" . | CHEMICAL ENGINEERING JOURNAL 454 (2023) . |
| APA | Cui, Yingchao , Gao, Jingfeng , Wu, Zejie , Wang, Zhiqi , Zhao, Yifan , Guo, Yi . Inhibition of Nitrospira and Nitrotoga by paracetamol achieved the rapid start-up and long-term stable operation of partial nitrification for low-strength ammonium wastewater . | CHEMICAL ENGINEERING JOURNAL , 2023 , 454 . |
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摘要 ：
本发明提出了一种同步脱氮去除难降解有机物的光芬顿废水处理方法和装置，所述方法包括如下步骤：将废水、H2O2加入到含有光芬顿催化剂和厌氧氨氧化菌的反应器中，在光照条件下进行反应，去除废水中的氮素和难降解有机物。本申请通过光芬顿实现难降解有机物的去除，通过以Anammox为核心的多物种群落脱氮并强化降碳，提出了一种更可持续的方法用于处理高含氮量、难降解有机物废水。
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| GB/T 7714 | 张莉 , 司光超 , 高景峰 et al. 同步脱氮去除难降解有机物的光芬顿废水处理方法和装置 : CN202310524995.0[P]. | 2023-05-10 . |
| MLA | 张莉 et al. "同步脱氮去除难降解有机物的光芬顿废水处理方法和装置" : CN202310524995.0. | 2023-05-10 . |
| APA | 张莉 , 司光超 , 高景峰 , 杨嘉春 . 同步脱氮去除难降解有机物的光芬顿废水处理方法和装置 : CN202310524995.0. | 2023-05-10 . |
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摘要 ：
一种利用塑料际富集硫自养反硝化菌并快速启动硫自养反硝化的方法，属于污水生物处理技术领域。本发明在硫自养反硝化系统中，选择序批式固定床生物膜反应器，将微塑料包固定在反应器中，培养微塑料生物膜(塑料际)。在硫自养反硝化菌成功在塑料际中富集后，将微塑料包取出，校验了其具有很高的硫自养反硝化活性。之后在不具有硫自养反硝化性能的活性污泥系统中接种塑料际，在较短的周期内实现了硫自养反硝化的启动和生物强化。且塑料际可以重复利用，具有现实意义且能解决污水深度处理的实际问题，易于应用和推广。
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| GB/T 7714 | 高景峰 , 王知其 , 王宇轩 et al. 一种利用塑料际富集硫自养反硝化菌并快速启动硫自养反硝化的方法 : CN202310030605.4[P]. | 2023-01-09 . |
| MLA | 高景峰 et al. "一种利用塑料际富集硫自养反硝化菌并快速启动硫自养反硝化的方法" : CN202310030605.4. | 2023-01-09 . |
| APA | 高景峰 , 王知其 , 王宇轩 , 袁钰焜 , 赵轶凡 , 王焓屹 . 一种利用塑料际富集硫自养反硝化菌并快速启动硫自养反硝化的方法 : CN202310030605.4. | 2023-01-09 . |
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摘要 ：
一种利用塑料际富集厌氧氨氧化菌并快速启动厌氧氨氧化过程的方法，属于污水生物处理技术领域。本发明在短程硝化耦合厌氧氨氧化系统中，选择序批式固定床生物膜反应器，培养微塑料生物膜(塑料际)。在厌氧氨氧化菌成功在微塑料表面富集后，将塑料包取出，验证了其具有较高的厌氧氨氧化活性。之后在不具有厌氧氨氧化性能的活性污泥系统中接种塑料际，在较短的周期内实现了厌氧氨氧化的启动和生物强化。且塑料际可以重复利用，易于在实际污水生物脱氮处理中应用和推广。
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| GB/T 7714 | 高景峰 , 王知其 , 王焓屹 et al. 一种利用塑料际富集厌氧氨氧化菌并快速启动厌氧氨氧化过程的方法 : CN202310030656.7[P]. | 2023-01-09 . |
| MLA | 高景峰 et al. "一种利用塑料际富集厌氧氨氧化菌并快速启动厌氧氨氧化过程的方法" : CN202310030656.7. | 2023-01-09 . |
| APA | 高景峰 , 王知其 , 王焓屹 , 张艺 , 赵轶凡 , 王宇轩 . 一种利用塑料际富集厌氧氨氧化菌并快速启动厌氧氨氧化过程的方法 : CN202310030656.7. | 2023-01-09 . |
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摘要 ：
The chloroxylenol (PCMX) degrading strain was successfully isolated from sludge and identified as Rhodococcus ruber (R. ruber). Afterwards, a bioaugmentation system was constructed by seeding R. ruber into nitrifying sludge to fasten degradation efficiency of highly toxic PCMX from wastewater. Results showed that R. ruber presented high PCMX-degrading performance under aerobic conditions, 25 degrees C, pH 7.0 and inoculum sizes of 4% (v/v). These optimized conditions were used in subsequent bioaugmentation experiment. In bioaugmentation system, R. ruber could detoxify nitrifiers by degrading PCMX, and the content of polysaccharide in extracellular poly-meric substances increased. The quantitative polymerase chain reaction results exhibited that the absolute abundance of 16S rRNA gene and ammonia oxidizing bacteria (AOB) slightly elevated in bioaugmentation system. After analyzing the results of high-throughput sequencing, it was found that the loaded R. ruber can colonize successfully and turn into dominant strains in sludge system. Molecular docking simulation showed that PCMX had a weaker suppressed effect on AOB than nitrite oxidizing bacteria, and R. ruber can alleviate the adverse effect. This study could provide a novel strategy for potential application in reinforcement of PCMX removal in wastewater treatment.
关键词 ：
Bacterial community Bacterial community Molecular docking simulation Molecular docking simulation Rhodococcus ruber Rhodococcus ruber Chloroxylenol Chloroxylenol Bioaugmentation Bioaugmentation
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| GB/T 7714 | Guo, Yi , Gao, Jingfeng , Zhao, Yifan et al. Mitigating the inhibition of antibacterial agent chloroxylenol on nitrification system-The role of Rhodococcus ruber in a bioaugmentation system [J]. | JOURNAL OF HAZARDOUS MATERIALS , 2023 , 447 . |
| MLA | Guo, Yi et al. "Mitigating the inhibition of antibacterial agent chloroxylenol on nitrification system-The role of Rhodococcus ruber in a bioaugmentation system" . | JOURNAL OF HAZARDOUS MATERIALS 447 (2023) . |
| APA | Guo, Yi , Gao, Jingfeng , Zhao, Yifan , Liu, Ying , Zhao, Mingyan , Li, Ziqiao . Mitigating the inhibition of antibacterial agent chloroxylenol on nitrification system-The role of Rhodococcus ruber in a bioaugmentation system . | JOURNAL OF HAZARDOUS MATERIALS , 2023 , 447 . |
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摘要 ：
Antibiotic resistant bacteria (ARB) and the antibiotic resistance genes (ARGs) dissemination via plasmid-mediated conjugation have attracted considerable attentions. In this research, sulfidated nanoscale zerovalent iron (S-nZVI)/peroxymonosulfate (PMS) and S-nZVI/peroxydisulfate (PDS) process were investigated to inactivate ARB (Escherichia coli DH5 alpha with RP4 plasmid, Pseudomonas. HLS-6 contains sul1 and intI1 on genome DNA sequence). S-nZVI/PMS system showed higher efficiency than S-nZVI/PDS on ARB inactivation. Thus, the optimal condition 28 mg/L S-nZVI coupled with 153.7 mg/L (0.5 mM) PMS was applied to remove both intracellular ARGs (iARGs) and ARB. The oxidative damage of ARB cell was systemically studied by cell viability, intracellular Mg2+ levels, the changes of extracellular and internal structure, integrity of cell walls and membranes and enzymatic activities. S-nZVI/PMS effectively inactivated ARB (similar to 7.32 log) within 15 min. These effects were greatly higher than those achieved individually. Moreover, removal efficiencies of iARGs sul1, intI1 and tetA were 1.52, 1.79 and 1.56 log, respectively. These results revealed that S-nZVI and PMS have a synergistic effect against ARB and iARGs. The regrowth assays illustrated that the ARB were effectively inactivated. By verifying the inhibitory impacts of S-nZVI/PMS treatment on conjugation transfer, this work highlights a promising alternative technique for inhibiting the horizontal gene transfer.
关键词 ：
Antibiotic resistant bacteria and genes Antibiotic resistant bacteria and genes Conjugative transfer Conjugative transfer Peroxydisulfate Peroxydisulfate Sulfidated nanoscale zerovalent iron Sulfidated nanoscale zerovalent iron Peroxymonosulfate Peroxymonosulfate
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| GB/T 7714 | Liu, Ying , Gao, Jingfeng , Wang, Yuwei et al. The removal of antibiotic resistant bacteria and genes and inhibition of the horizontal gene transfer by contrastive research on sulfidated nanoscale zerovalent iron activating peroxymonosulfate or peroxydisulfate [J]. | JOURNAL OF HAZARDOUS MATERIALS , 2022 , 423 . |
| MLA | Liu, Ying et al. "The removal of antibiotic resistant bacteria and genes and inhibition of the horizontal gene transfer by contrastive research on sulfidated nanoscale zerovalent iron activating peroxymonosulfate or peroxydisulfate" . | JOURNAL OF HAZARDOUS MATERIALS 423 (2022) . |
| APA | Liu, Ying , Gao, Jingfeng , Wang, Yuwei , Duan, Wanjun , Liu, Jie , Zhang, Yi et al. The removal of antibiotic resistant bacteria and genes and inhibition of the horizontal gene transfer by contrastive research on sulfidated nanoscale zerovalent iron activating peroxymonosulfate or peroxydisulfate . | JOURNAL OF HAZARDOUS MATERIALS , 2022 , 423 . |
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摘要 ：
Triclocarban (TCC) is in great market demand especially after the outbreak of COVID-19 pandemic, becoming an emerging pollutant. However, the impacts of TCC on the performance of nitrifying granular sludge system and the occurrence of antibiotic resistance genes (ARGs) were still unknown. This work explored the impacts of different concentrations of TCC on nitrifying granular sludge. Results showed that TCC suppressed the activities of ammonia-oxidizing microorganisms and decreased the abundance of Nitrospira. Adsorption was the main way for the removal of TCC and the biodegradation efficiency of TCC increased to 28.00% under 19.70 mg/L TCC addition. TCC enriched the ARGs and promoted the risks of their transferring in microorganisms. Pseudomonas might not only have strong resistance to TCC, but also propagate ARGs. The removal process of TCC and bacterial communities were important factors to promote the spread of ARGs. Thus, the existence of TCC presented a great environmental risk.
关键词 ：
Antibiotic resistance genes Antibiotic resistance genes Nitrifying granular sludge performance Nitrifying granular sludge performance Bacterial community structure Bacterial community structure Triclocarban Triclocarban
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| GB/T 7714 | Wang, Zhiqi , Gao, Jingfeng , Wang, Shijie et al. Triclocarban shifted the microbial communities and promoted the spread of antibiotic resistance genes in nitrifying granular sludge system [J]. | BIORESOURCE TECHNOLOGY , 2022 , 347 . |
| MLA | Wang, Zhiqi et al. "Triclocarban shifted the microbial communities and promoted the spread of antibiotic resistance genes in nitrifying granular sludge system" . | BIORESOURCE TECHNOLOGY 347 (2022) . |
| APA | Wang, Zhiqi , Gao, Jingfeng , Wang, Shijie , Zhao, Yifan , Dai, Huihui , Li, Dingchang et al. Triclocarban shifted the microbial communities and promoted the spread of antibiotic resistance genes in nitrifying granular sludge system . | BIORESOURCE TECHNOLOGY , 2022 , 347 . |
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摘要 ：
Chloroxylenol (PCMX), an antibacterial agent, has been widely detected in water environment and has toxic effects on biology and ecology. During 270 d, the influence of PCMX on the performance of three nitrification systems was investigated, including floc-based sequencing batch reactor (FSBR), aerobic granule-based SBR (AGSBR) and biofilm SBR (BSBR). The nitrification capability of three systems was inhibited by PCMX, but recovered after domestication, and PCMX made three systems realize partial nitrification for 10, 100 and 35 days, respectively. The extracellular polymeric substances of three systems increased first and then decreased with the increment of PCMX. The granular structure of AGSBR may be conducive to the enrichment of antibiotic resistance genes (ARGs), and almost all ARGs of BSBR were reduced during the addition of 5.0 mg/L PCMX. The microbial community results showed that Rhodococcus as potential degrading bacteria was continuously enriched in three systems. Piscinibacter was regarded as the potential antibiotic resistant bacteria, which was positively associated with multiple ARGs in three systems. Additionally, quaternary ammonium compounds resistance
关键词 ：
Mar?a Sonia Rodr?guez-Cruz Mar?a Sonia Rodr?guez-Cruz Partial nitrification Partial nitrification Chloroxylenol Chloroxylenol Bacterial community Bacterial community Editor Editor Three forms of sludge Three forms of sludge Antibiotic resistance genes Antibiotic resistance genes
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| GB/T 7714 | Guo, Yi , Gao, Jingfeng , Wang, Zhiqi et al. The fate and behavior mechanism of antibiotic resistance genes and microbial communities in flocs, aerobic granular and biofilm sludge under chloroxylenol pressure [J]. | JOURNAL OF HAZARDOUS MATERIALS , 2022 , 438 . |
| MLA | Guo, Yi et al. "The fate and behavior mechanism of antibiotic resistance genes and microbial communities in flocs, aerobic granular and biofilm sludge under chloroxylenol pressure" . | JOURNAL OF HAZARDOUS MATERIALS 438 (2022) . |
| APA | Guo, Yi , Gao, Jingfeng , Wang, Zhiqi , Cui, Yingchao , Li, Ziqiao , Wu, Zejie et al. The fate and behavior mechanism of antibiotic resistance genes and microbial communities in flocs, aerobic granular and biofilm sludge under chloroxylenol pressure . | JOURNAL OF HAZARDOUS MATERIALS , 2022 , 438 . |
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摘要 ：
Metagenomic approach was applied to simultaneously reveal the antibiotic resistance genes (ARGs) and antibacterial biocide & metal resistance genes (BMRGs), and the corresponding microbial hosts with high mobility during aerobic granular sludge (AGS) formation process. The results showed that the relative abundance of BMRGs was 88-123 times that of ARGs. AGS process was easier to enrich BMRGs, leading to a greater risk of drug resistance caused by BMRGs than that by ARGs. The enrichments of ARGs and BMRGs in AGS were closely related to several enhanced microbial metabolisms (i.e., cell motility, transposase and ATP-binding cassette transporters) and their corresponding regulatory genes. Several enhanced KEGG Orthologs (KO) functions, such as K01995, K01996, K01997 and K02002, might cause a positive impact on the spread of ARGs and BMRGs, and the main contributors were the largely enriched glycogens accumulating organisms. The first dominant ARGs (adeF) was carried by lots of microbial hosts, which might be enriched and propagated mainly through horizontal gene transfer. Candidatus Competibacter denitrificans simultaneously harbored ARG (cmx) and Cu related RGs (corR). Many enriched bacteria contained simultaneously multiple BMRGs (copR and corR) and mobile genetic elements (integrons and plasmids), granting them high mobility capabilities and contributing to the spread of BMRGs. This study might provide deeper understandings of the proliferation and mobility of ARGs and BMRGs, importantly, highlighted the status of BMRGs, which laid the foundation for the controlling widespread of resistance genes in AGS.
关键词 ：
Aerobic granular sludge Aerobic granular sludge Horizontal gene transfer Horizontal gene transfer Co-selection Co-selection Metagenomic sequencing Metagenomic sequencing Antibacterial biocide & metal resistance genes Antibacterial biocide & metal resistance genes Antibiotic resistance genes Antibiotic resistance genes
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| GB/T 7714 | Li, Dingchang , Gao, Jingfeng , Dai, Huihui et al. Higher spreading risk of antibacterial biocide and heavy metal resistance genes than antibiotic resistance genes in aerobic granular sludge [J]. | ENVIRONMENTAL RESEARCH , 2022 , 212 . |
| MLA | Li, Dingchang et al. "Higher spreading risk of antibacterial biocide and heavy metal resistance genes than antibiotic resistance genes in aerobic granular sludge" . | ENVIRONMENTAL RESEARCH 212 (2022) . |
| APA | Li, Dingchang , Gao, Jingfeng , Dai, Huihui , Wang, Zhiqi , Zhao, Yifan , Cui, Yingchao . Higher spreading risk of antibacterial biocide and heavy metal resistance genes than antibiotic resistance genes in aerobic granular sludge . | ENVIRONMENTAL RESEARCH , 2022 , 212 . |
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